primary antibodies targeting igfbp2 Search Results


rat α mouse  (R&D Systems)
92
R&D Systems rat α mouse
Rat α Mouse, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rat α mouse/product/R&D Systems
Average 92 stars, based on 1 article reviews
rat α mouse - by Bioz Stars, 2026-03
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gene exp igfbp2 hs01040719 m1  (Thermo Fisher)
85
Thermo Fisher gene exp igfbp2 hs01040719 m1
Gene Exp Igfbp2 Hs01040719 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene exp igfbp2 hs01040719 m1/product/Thermo Fisher
Average 85 stars, based on 1 article reviews
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igfbp 2 shrna plasmid control sc 108060 puromycin  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology igfbp 2 shrna plasmid control sc 108060 puromycin
Igfbp 2 Shrna Plasmid Control Sc 108060 Puromycin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/igfbp 2 shrna plasmid control sc 108060 puromycin/product/Santa Cruz Biotechnology
Average 96 stars, based on 1 article reviews
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igfbp2 c-18 antibody  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology igfbp2 c-18 antibody
Igfbp2 C 18 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/igfbp2 c-18 antibody/product/Santa Cruz Biotechnology
Average 90 stars, based on 1 article reviews
igfbp2 c-18 antibody - by Bioz Stars, 2026-03
90/100 stars
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gene exp igfbp2 mm00492632 m1  (Thermo Fisher)
89
Thermo Fisher gene exp igfbp2 mm00492632 m1
Assessment of protein levels in conditioned media from primary fibroblast culture. (A) Representative blots of relevant cytokines, chemokines, and growth factors from RayBiotech Mouse Cytokine Antibody Arrays demonstrating WT and Bg fibroblasts’ relative protein secretion in conditioned media. Fibroblasts lines were derived from 2 to 3 mice from each genotype and experiments were done with 3–5 technical replicates. (B) Quantification of the integrated densities (n = 3 per group). Integrated densities are relative to manufacturer controls. Relative integrated densities of MCP-1, IGF-1, <t>and</t> <t>IGFBP-2</t> were significantly decreased in Bg samples (p = 0.0083, p = 0.0159, and p = 0.0111, respectively). (C) Confirmatory ELISAs of MCP-1 and IGFBP-2 (n = 5 per group) show decreased protein secretion from Bg fibroblasts (p = 0.0068 and p = 0.0498, respectively). *p ≤ 0.05, **p ≤ 0.01
Gene Exp Igfbp2 Mm00492632 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene exp igfbp2 mm00492632 m1/product/Thermo Fisher
Average 89 stars, based on 1 article reviews
gene exp igfbp2 mm00492632 m1 - by Bioz Stars, 2026-03
89/100 stars
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igfbp2 polyclonal antibody  (Bioss)
93
Bioss igfbp2 polyclonal antibody
Assessment of protein levels in conditioned media from primary fibroblast culture. (A) Representative blots of relevant cytokines, chemokines, and growth factors from RayBiotech Mouse Cytokine Antibody Arrays demonstrating WT and Bg fibroblasts’ relative protein secretion in conditioned media. Fibroblasts lines were derived from 2 to 3 mice from each genotype and experiments were done with 3–5 technical replicates. (B) Quantification of the integrated densities (n = 3 per group). Integrated densities are relative to manufacturer controls. Relative integrated densities of MCP-1, IGF-1, <t>and</t> <t>IGFBP-2</t> were significantly decreased in Bg samples (p = 0.0083, p = 0.0159, and p = 0.0111, respectively). (C) Confirmatory ELISAs of MCP-1 and IGFBP-2 (n = 5 per group) show decreased protein secretion from Bg fibroblasts (p = 0.0068 and p = 0.0498, respectively). *p ≤ 0.05, **p ≤ 0.01
Igfbp2 Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/igfbp2 polyclonal antibody/product/Bioss
Average 93 stars, based on 1 article reviews
igfbp2 polyclonal antibody - by Bioz Stars, 2026-03
93/100 stars
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rabbit antihuman igfbp-2  (Upstate Biotechnology Inc)
90
Upstate Biotechnology Inc rabbit antihuman igfbp-2
Assessment of protein levels in conditioned media from primary fibroblast culture. (A) Representative blots of relevant cytokines, chemokines, and growth factors from RayBiotech Mouse Cytokine Antibody Arrays demonstrating WT and Bg fibroblasts’ relative protein secretion in conditioned media. Fibroblasts lines were derived from 2 to 3 mice from each genotype and experiments were done with 3–5 technical replicates. (B) Quantification of the integrated densities (n = 3 per group). Integrated densities are relative to manufacturer controls. Relative integrated densities of MCP-1, IGF-1, <t>and</t> <t>IGFBP-2</t> were significantly decreased in Bg samples (p = 0.0083, p = 0.0159, and p = 0.0111, respectively). (C) Confirmatory ELISAs of MCP-1 and IGFBP-2 (n = 5 per group) show decreased protein secretion from Bg fibroblasts (p = 0.0068 and p = 0.0498, respectively). *p ≤ 0.05, **p ≤ 0.01
Rabbit Antihuman Igfbp 2, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit antihuman igfbp-2/product/Upstate Biotechnology Inc
Average 90 stars, based on 1 article reviews
rabbit antihuman igfbp-2 - by Bioz Stars, 2026-03
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mouse igfbp-2 biotinylated antibody  (Bio-Techne corporation)
90
Bio-Techne corporation mouse igfbp-2 biotinylated antibody
Assessment of protein levels in conditioned media from primary fibroblast culture. (A) Representative blots of relevant cytokines, chemokines, and growth factors from RayBiotech Mouse Cytokine Antibody Arrays demonstrating WT and Bg fibroblasts’ relative protein secretion in conditioned media. Fibroblasts lines were derived from 2 to 3 mice from each genotype and experiments were done with 3–5 technical replicates. (B) Quantification of the integrated densities (n = 3 per group). Integrated densities are relative to manufacturer controls. Relative integrated densities of MCP-1, IGF-1, <t>and</t> <t>IGFBP-2</t> were significantly decreased in Bg samples (p = 0.0083, p = 0.0159, and p = 0.0111, respectively). (C) Confirmatory ELISAs of MCP-1 and IGFBP-2 (n = 5 per group) show decreased protein secretion from Bg fibroblasts (p = 0.0068 and p = 0.0498, respectively). *p ≤ 0.05, **p ≤ 0.01
Mouse Igfbp 2 Biotinylated Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse igfbp-2 biotinylated antibody/product/Bio-Techne corporation
Average 90 stars, based on 1 article reviews
mouse igfbp-2 biotinylated antibody - by Bioz Stars, 2026-03
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human igfbp-2 quantikine elisa kit  (Bio-Techne corporation)
94
Bio-Techne corporation human igfbp-2 quantikine elisa kit
Assessment of protein levels in conditioned media from primary fibroblast culture. (A) Representative blots of relevant cytokines, chemokines, and growth factors from RayBiotech Mouse Cytokine Antibody Arrays demonstrating WT and Bg fibroblasts’ relative protein secretion in conditioned media. Fibroblasts lines were derived from 2 to 3 mice from each genotype and experiments were done with 3–5 technical replicates. (B) Quantification of the integrated densities (n = 3 per group). Integrated densities are relative to manufacturer controls. Relative integrated densities of MCP-1, IGF-1, <t>and</t> <t>IGFBP-2</t> were significantly decreased in Bg samples (p = 0.0083, p = 0.0159, and p = 0.0111, respectively). (C) Confirmatory ELISAs of MCP-1 and IGFBP-2 (n = 5 per group) show decreased protein secretion from Bg fibroblasts (p = 0.0068 and p = 0.0498, respectively). *p ≤ 0.05, **p ≤ 0.01
Human Igfbp 2 Quantikine Elisa Kit, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human igfbp-2 quantikine elisa kit/product/Bio-Techne corporation
Average 94 stars, based on 1 article reviews
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94/100 stars
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mouse igfbp-2 duoset elisa  (Bio-Techne corporation)
93
Bio-Techne corporation mouse igfbp-2 duoset elisa
Assessment of protein levels in conditioned media from primary fibroblast culture. (A) Representative blots of relevant cytokines, chemokines, and growth factors from RayBiotech Mouse Cytokine Antibody Arrays demonstrating WT and Bg fibroblasts’ relative protein secretion in conditioned media. Fibroblasts lines were derived from 2 to 3 mice from each genotype and experiments were done with 3–5 technical replicates. (B) Quantification of the integrated densities (n = 3 per group). Integrated densities are relative to manufacturer controls. Relative integrated densities of MCP-1, IGF-1, <t>and</t> <t>IGFBP-2</t> were significantly decreased in Bg samples (p = 0.0083, p = 0.0159, and p = 0.0111, respectively). (C) Confirmatory ELISAs of MCP-1 and IGFBP-2 (n = 5 per group) show decreased protein secretion from Bg fibroblasts (p = 0.0068 and p = 0.0498, respectively). *p ≤ 0.05, **p ≤ 0.01
Mouse Igfbp 2 Duoset Elisa, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse igfbp-2 duoset elisa/product/Bio-Techne corporation
Average 93 stars, based on 1 article reviews
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igfbp2 22-bp2hu-e01  (ALPCO)
90
ALPCO igfbp2 22-bp2hu-e01
Assessment of protein levels in conditioned media from primary fibroblast culture. (A) Representative blots of relevant cytokines, chemokines, and growth factors from RayBiotech Mouse Cytokine Antibody Arrays demonstrating WT and Bg fibroblasts’ relative protein secretion in conditioned media. Fibroblasts lines were derived from 2 to 3 mice from each genotype and experiments were done with 3–5 technical replicates. (B) Quantification of the integrated densities (n = 3 per group). Integrated densities are relative to manufacturer controls. Relative integrated densities of MCP-1, IGF-1, <t>and</t> <t>IGFBP-2</t> were significantly decreased in Bg samples (p = 0.0083, p = 0.0159, and p = 0.0111, respectively). (C) Confirmatory ELISAs of MCP-1 and IGFBP-2 (n = 5 per group) show decreased protein secretion from Bg fibroblasts (p = 0.0068 and p = 0.0498, respectively). *p ≤ 0.05, **p ≤ 0.01
Igfbp2 22 Bp2hu E01, supplied by ALPCO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/igfbp2 22-bp2hu-e01/product/ALPCO
Average 90 stars, based on 1 article reviews
igfbp2 22-bp2hu-e01 - by Bioz Stars, 2026-03
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duoset elisa kit  (R&D Systems)
93
R&D Systems duoset elisa kit
Assessment of protein levels in conditioned media from primary fibroblast culture. (A) Representative blots of relevant cytokines, chemokines, and growth factors from RayBiotech Mouse Cytokine Antibody Arrays demonstrating WT and Bg fibroblasts’ relative protein secretion in conditioned media. Fibroblasts lines were derived from 2 to 3 mice from each genotype and experiments were done with 3–5 technical replicates. (B) Quantification of the integrated densities (n = 3 per group). Integrated densities are relative to manufacturer controls. Relative integrated densities of MCP-1, IGF-1, <t>and</t> <t>IGFBP-2</t> were significantly decreased in Bg samples (p = 0.0083, p = 0.0159, and p = 0.0111, respectively). (C) Confirmatory ELISAs of MCP-1 and IGFBP-2 (n = 5 per group) show decreased protein secretion from Bg fibroblasts (p = 0.0068 and p = 0.0498, respectively). *p ≤ 0.05, **p ≤ 0.01
Duoset Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/duoset elisa kit/product/R&D Systems
Average 93 stars, based on 1 article reviews
duoset elisa kit - by Bioz Stars, 2026-03
93/100 stars
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Image Search Results


Assessment of protein levels in conditioned media from primary fibroblast culture. (A) Representative blots of relevant cytokines, chemokines, and growth factors from RayBiotech Mouse Cytokine Antibody Arrays demonstrating WT and Bg fibroblasts’ relative protein secretion in conditioned media. Fibroblasts lines were derived from 2 to 3 mice from each genotype and experiments were done with 3–5 technical replicates. (B) Quantification of the integrated densities (n = 3 per group). Integrated densities are relative to manufacturer controls. Relative integrated densities of MCP-1, IGF-1, and IGFBP-2 were significantly decreased in Bg samples (p = 0.0083, p = 0.0159, and p = 0.0111, respectively). (C) Confirmatory ELISAs of MCP-1 and IGFBP-2 (n = 5 per group) show decreased protein secretion from Bg fibroblasts (p = 0.0068 and p = 0.0498, respectively). *p ≤ 0.05, **p ≤ 0.01

Journal: Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society

Article Title: The lysosomal trafficking regulator is necessary for normal wound healing

doi: 10.1111/wrr.12984

Figure Lengend Snippet: Assessment of protein levels in conditioned media from primary fibroblast culture. (A) Representative blots of relevant cytokines, chemokines, and growth factors from RayBiotech Mouse Cytokine Antibody Arrays demonstrating WT and Bg fibroblasts’ relative protein secretion in conditioned media. Fibroblasts lines were derived from 2 to 3 mice from each genotype and experiments were done with 3–5 technical replicates. (B) Quantification of the integrated densities (n = 3 per group). Integrated densities are relative to manufacturer controls. Relative integrated densities of MCP-1, IGF-1, and IGFBP-2 were significantly decreased in Bg samples (p = 0.0083, p = 0.0159, and p = 0.0111, respectively). (C) Confirmatory ELISAs of MCP-1 and IGFBP-2 (n = 5 per group) show decreased protein secretion from Bg fibroblasts (p = 0.0068 and p = 0.0498, respectively). *p ≤ 0.05, **p ≤ 0.01

Article Snippet: Taqman Fast Advanced Master Mix (Cat# 4444557, Thermo Fisher Scientific) with the following hybridization probes were utilised for qPCR of cDNA samples: LYST (Cat# Mm00465000_m1), CCL2 (Cat# Mm00441242_m1), IGF1 (Cat# Mm00439560_m1), IGFBP2 (Cat# Mm00492632_m1), Col1a1 (Cat# Mm00801666_g1), Col1a2 (Cat# Mm00483888_m1), and Col3a1 (Mm00802331_m1).

Techniques: Derivative Assay

Protein localization and gene expression in fibroblasts. (A) LAMP-1 immunofluorescent (IF) images of WT and Bg fibroblasts demonstrate enlarged lysosomes in Bg fibroblasts. (B) RT-qPCR analysis shows no change in relative gene expression of lysosomal trafficking regulator (LYST) between WT and Bg fibroblasts. (C) MCP-1 staining shows primarily cytoplasmic staining of similar degree between genotypes. (D) Gene expression of MCP-1 is comparable between genotypes at all time points. (E) Representative images of IGF-1 also show high nuclear localization with presence in nucleoli in both genotypes. (F) IGF-1 gene expression between cell types is similar at 24 and 48 h. At 72 h, Bg cells show a higher degree of gene expression (p = 0.0156). (G) Representative IF images of IGFBP-2 show a high degree of nuclear localization in both genotypes. (H) Relative gene expression of IGFBP-2 is decreased in Bg fibroblasts at 24 h (p = 0.0083) but is comparable to WT cells starting at 48 h. Fibroblasts lines were derived from 2 to 3 mice from each genotype and experiments were done with 5–6 technical replicates. DAPI was used to visualise nuclei (blue). Actin is represented with green, and proteins of interest are red. Scale bar = 20 μm. *p ≤ 0.05, **p ≤ 0.01 (n = 5–6 per group)

Journal: Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society

Article Title: The lysosomal trafficking regulator is necessary for normal wound healing

doi: 10.1111/wrr.12984

Figure Lengend Snippet: Protein localization and gene expression in fibroblasts. (A) LAMP-1 immunofluorescent (IF) images of WT and Bg fibroblasts demonstrate enlarged lysosomes in Bg fibroblasts. (B) RT-qPCR analysis shows no change in relative gene expression of lysosomal trafficking regulator (LYST) between WT and Bg fibroblasts. (C) MCP-1 staining shows primarily cytoplasmic staining of similar degree between genotypes. (D) Gene expression of MCP-1 is comparable between genotypes at all time points. (E) Representative images of IGF-1 also show high nuclear localization with presence in nucleoli in both genotypes. (F) IGF-1 gene expression between cell types is similar at 24 and 48 h. At 72 h, Bg cells show a higher degree of gene expression (p = 0.0156). (G) Representative IF images of IGFBP-2 show a high degree of nuclear localization in both genotypes. (H) Relative gene expression of IGFBP-2 is decreased in Bg fibroblasts at 24 h (p = 0.0083) but is comparable to WT cells starting at 48 h. Fibroblasts lines were derived from 2 to 3 mice from each genotype and experiments were done with 5–6 technical replicates. DAPI was used to visualise nuclei (blue). Actin is represented with green, and proteins of interest are red. Scale bar = 20 μm. *p ≤ 0.05, **p ≤ 0.01 (n = 5–6 per group)

Article Snippet: Taqman Fast Advanced Master Mix (Cat# 4444557, Thermo Fisher Scientific) with the following hybridization probes were utilised for qPCR of cDNA samples: LYST (Cat# Mm00465000_m1), CCL2 (Cat# Mm00441242_m1), IGF1 (Cat# Mm00439560_m1), IGFBP2 (Cat# Mm00492632_m1), Col1a1 (Cat# Mm00801666_g1), Col1a2 (Cat# Mm00483888_m1), and Col3a1 (Mm00802331_m1).

Techniques: Gene Expression, Quantitative RT-PCR, Staining, Derivative Assay

Localization of IGF associated proteins in fibroblasts. (A) Co-stain of IGF-1 (red) and IGFBP-2 (green) shows nuclear colocalization (yellow) of the proteins based on the microscopic resolution (0.24 μm). Colocalization is not seen in the cytoplasm or in the nucleoli of the nucleus. (B) IGF-1 (red) and IGF-1 receptor (IGF-1R) (green) do not appear to colocalize. IGF-1R appears mostly cytoplasmic. (C) Lysosomes stained with LAMP-1 (red) and IGF-1R (green) show colocalization associated with the membrane of lysosomes. DAPI (blue) is used to visualise nuclei and void regions in nuclei are nucleoli. Scale bar = 20 μm

Journal: Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society

Article Title: The lysosomal trafficking regulator is necessary for normal wound healing

doi: 10.1111/wrr.12984

Figure Lengend Snippet: Localization of IGF associated proteins in fibroblasts. (A) Co-stain of IGF-1 (red) and IGFBP-2 (green) shows nuclear colocalization (yellow) of the proteins based on the microscopic resolution (0.24 μm). Colocalization is not seen in the cytoplasm or in the nucleoli of the nucleus. (B) IGF-1 (red) and IGF-1 receptor (IGF-1R) (green) do not appear to colocalize. IGF-1R appears mostly cytoplasmic. (C) Lysosomes stained with LAMP-1 (red) and IGF-1R (green) show colocalization associated with the membrane of lysosomes. DAPI (blue) is used to visualise nuclei and void regions in nuclei are nucleoli. Scale bar = 20 μm

Article Snippet: Taqman Fast Advanced Master Mix (Cat# 4444557, Thermo Fisher Scientific) with the following hybridization probes were utilised for qPCR of cDNA samples: LYST (Cat# Mm00465000_m1), CCL2 (Cat# Mm00441242_m1), IGF1 (Cat# Mm00439560_m1), IGFBP2 (Cat# Mm00492632_m1), Col1a1 (Cat# Mm00801666_g1), Col1a2 (Cat# Mm00483888_m1), and Col3a1 (Mm00802331_m1).

Techniques: Staining, Membrane